Category: Enzymes & Accessories
MEDNA BirA is N-terminal tagged with FLAG-myc-His-MBP to provide the convenience to remove the enzyme after biotinylation. MEDNA BirA enzyme is produced in E. coli and purified to greater than 95% purity by affinity purification. The multiple affinity tags and the high purity make this enzyme desirable over others available in the market.
Biotin binds to avidin or streptavidin very tightly, and this interaction has been used in many applications. BirA biotin-protein ligase (EC 22.214.171.124) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to a lysine residue in a biotin-accepting polypeptide (such as the AviTag™ peptide). The protein is encoded by the birA gene.