M1 DNA Polymerase (Taq)
Category: Enzymes & Accessories
MEDNA M1 DNA polymerase (Taq) is a recombinant and thermostable Taq polymerase (expressed from the Thermus aquaticus gene, BRENDA: EC 22.214.171.124) expressed in E.coli. The enzyme is robust in amplifying DNA fragments from 100 bp to 10 kb. By the addition of A to the 3' end of the PCR product, the product can be cloned into TA vector efficiently.
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity. M1 DNA polymerase is used to amplify DNA in the polymerase chain reaction (PCR). It can also be used in gene amplification, sequencing and genotyping applications.
Supplied with 10x Taq Pol II Reaction Buffer with or without 2.5 mM MgCl2. Concentration: 5 units/µL.
Unit definition: One unit will incorporate 10 nmols of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.