M5 DNA Polymerase (pfu)
Category: Enzymes & Accessories
M5 DNA polymerase (pfu) is an engineered, high-fidelity DNA polymerase from Pyrococcus furiosus recombinantly expressed in E. coli. It is used for high fidelity applications due to its 3´→5´ exonuclease (proofreading) activity.
Pfu DNA Polymerase is a thermostable enzyme that has optimum activity at 75℃ in the presence of magnesium. It has greater than 10x fidelity than Taq DNA polymerase (Lundberg et al., 1991, Gene, 108(1):1-6).
20 mM Tris pH 7.9, 100 mM KCl, 0.1% Triton X-100, 0.1 mM EDTA, 1 mM DTT, 50% glycerol. 0.2 µm filtered solution.
Conventional Pfu Polymerase PCR ingredients, buffers, conditions and protocols will apply.
Storage at -80℃. Reconstituted protein solution can be stored at 2-8ºC for 7-14 days. Avoid multiple freeze-thaw cycles.