M5 DNA Polymerase (pfu)

Category: Enzymes & Accessories

Catalog # Size Price Quantity
M3105 100 units $108
M3105 500 units $432


M5 DNA polymerase (pfu) is an engineered, high-fidelity DNA polymerase from Pyrococcus furiosus recombinantly expressed in E. coli. It is used for high fidelity applications due to its 3´→5´ exonuclease (proofreading) activity.



Pfu DNA Polymerase is a thermostable enzyme that has optimum activity at 75 in the presence of magnesium. It has greater than 10x fidelity than Taq DNA polymerase (Lundberg et al., 1991, Gene, 108(1):1-6).



20 mM Tris pH 7.9, 100 mM KCl, 0.1% Triton X-100, 0.1 mM EDTA, 1 mM DTT, 50% glycerol. 0.2 µm filtered solution.



Conventional Pfu Polymerase PCR ingredients, buffers, conditions and protocols will apply.



Storage at -80℃. Reconstituted protein solution can be stored at 2-8ºC for 7-14 days. Avoid multiple freeze-thaw cycles.



Purity: ≥85%.