M19 Recombinant RNase A

Category: Enzymes & Accessories

Catalog # Size Price Quantity
M3119 200 µg $159
M3119 1 mg $550

DESCRIPTION:

M19 RNase A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. M19 Rnase A is recombinantly expressed in and purified from E. coli.

 

BACKGROUND:

M19 Ribonuclease A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.

 

FORMULATION:

20 mM Tris pH 8.0. 0.2 µm filtered solution.

 

ACTIVITY/USAGE:

1,000 U/mL. Use on clarified lysate at a final concentration of 100 U/mL for 30 min at room temperature.

 

APPLICATIONS:  Removal of RNA during plasmid DNA purification. Removal of unspecifically bound RNA. RNAse protection assays. Analysis RNA sequences. Hydrolysis of RNA in protein samples. 

 

STORAGE: -80

 

QC: ˃95% purity by SDS densitometry.