SARS-CoV-2 ELISA Key Reagent Kit
Category: Proteins & Antibodies
|D3201||Sufficient for 5 microplates||$1200|
|D3201||Sufficient for 20 microplates||$3648|
|D3201||Sufficient for 50 microplates||$8640|
Coronavirus disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). When humans are exposed to the virus, they can acquire immunity by forming IgG antibodies against the spike protein on the viral envelope. It is vital to know which individuals are already exposed to the virus by testing for the human IgGs that are present in the serum against the viral spike protein. This reagent kit is developed to help develop other diagnostic tests and assays to detect the human IgGs against SARS-CoV-2 in the serum. The kit contains the key reagents necessary (spike protein, antibodies, and binding standard), and these reagents can be used with any ELISA-compatible microplates, and other generic buffers and reagents for HRP detection.
This assay employs the quantitative sandwich enzyme immunoassay technique. The user-provided 96-well plates are coated with the capturing spike protein provided, The samples and standards are applied to the wells. The detecting antibody is used to detect the signal from the human IgG Fc in the samples. More details are provided in the protocol booklet.
Key components included in the kit:
1. Capturing protein – SARS-CoV-2 spike
2. Detecting antibody – HuFc Detection Antibody
3. Binding standard and positive control.
The following reagents are NOT included in the kit and must be provided by the end users:
1. 96-well or 384-well ELISA
2. Assay Diluent (PBS + 1% BSA)
3. Wash buffer (PBS + 0.01% Tween-20)
4. HRP substrate (TMB)
5. Stop solution (0.5N HCl)
6. Absorbance microplate reader (we recommend BMG LABTECH microplate readers)
ELISA assay reactivity is sensitive to
variations in operator, pipetting and washing techniques, incubation time,
temperature, composition of reagents, and other experimental variables. Assay
optimization may be required to generate the standard curve and fit the samples
in the specified detection range.
**Application for Emergence Use Authorization by FDA for diagnostic purposes is in process.