Biotinylated Monoclonal Anti-His Tag IgG is intended to be used in western blot, ELISA and IHC to detect His
Tag proteins. The monoclonal anti His Tag antibody is biotinylated at optimal condition to allow high sensitivity
and an excellent signal-to-noise ratio.
- High sensitivity
- Excellent signal-to-noise ratio
For the western blot the recommended final concentration range for use is 1-5 µg/mL.
If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous
immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum
from the same species as the tissue. The recommended concentration range for use is 2-10 µg/mL.
2° - 8ºC