HF502 Cell Culture Feed

Category: Media & Buffers

Catalog # Size Price Quantity
C1604 50 mL $100

DESCRIPTION

High protein yield feed HF502 is a liquid nutrient supplement. It is an animal-origin-free partial-feed formulation for CHO cells.
When combined with HF511 (Catalog Number C1603) it provides up to 10 fold improvement in titers of fed batch versus batch culture.


RECOMMENDATION OF FEEDING

During a typical fed batch protein production, four doses of feeds are required: the first feed is on day 3 post-seeding followed by 3 additional feeds every 2 days with 0.2% HF502 (v/v) + 2.5% HF511 (v/v). Glucose needs to be added when the level of glucose is low. Temperature shift may be applied.


OSMOLALITY

2400 +/- 400

pH
>12


STERILITY

Negative


STORAGE

Store at 2°- 8ºC protected from light.