HF511 Cell Culture Feed

Category: Media & Buffers

Catalog # Size Price Quantity
C1603 500 mL $500

DESCRIPTION

High protein yield feed HF511 is a liquid nutrient supplement. It is an animal-origin free partial feed formulation for CHO cells. When combined with HF502 (Catalog Number C1604) it provides up to 10 fold improvement in titers of feed batch versus batch culture.

RECOMMENDATION OF FEEDING

During a typical fed-batch protein production, four doses of feeds are required: the first feed is on day 3 post-seeding followed with 3 additional feeds every 2 days with 0.2% HF502 (v/v) + 2.5% HF511 (v/v). Glucose needs to be added when level of glucose is low. Temperature shift may be applied.

OSMOLALITY

2400 +/- 400

pH

5.0-6.0

STERILITY

Negative

STORAGE

Store at 2°- 8°C protected from light